Estonia's Anett Kontaveit became the highest seed to be knocked out of Wimbledon so far as she was beaten in straight sets by German Jule Niemeier.
World number 97 Niemeier overpowered the second seed to win 6-4 6-0.
"Amazing. I am speechless to be honest," said the 22-year-old Niemeier after reaching the third round.
Garbine Muguruza also made an early exit - the Spanish ninth seed, who won the title in 2017, was beaten 6-4 6-0 by Belgium's Greet Minnen.
Kontaveit, 26, has spoken about suffering from the longer-term effects of Covid and arrived at SW19 having not played since the French Open in May.
Niemeier added: "It is one of the biggest wins of my career so far.
"Winning at Wimbledon on Court One is an amazing feeling. I am happy I could handle the situation today. I love playing on grass."
Meanwhile, sixth seed Karolina Pliskova finished what she started on Tuesday night by defeating fellow Czech Tereza Martincova after the resumption of their match.
The 30-year-old, who was beaten by Ashleigh Barty in last year's final, won 7-6 (7-1) 7-5 in one hour and 42 minutes.