Gloucester-Hartpury overcame Exeter Chiefs 34-19 to become Premier 15s champions for the first time.
Tries from Kelsey Jones, Rachel Lund and Sarah Beckett gave Gloucester-Hartpury a 17-7 half-time lead, with Emily Tuttosi scoring for Exeter.
A penalty try after Claudia MacDonald slapped the ball away from danger extended the Cherry and Whites' lead.
Liv McGoverne's score gave Exeter hope but Lisa Neumann strolled over to confirm Gloucester-Hartpury's win.
Ebony Jeffries touched down with a late consolation for the visitors but it was the Cherry and Whites who celebrated at the final whistle.
Sean Lynn's side, who finished top of the league in the regular season, triumphed in front of a record crowd for the showpiece of 9,668 - in what was the first final played by two teams from outside London.
Gloucester's Kingsholm Stadium, chosen as the venue during the campaign, was rebranded as 'Queensholm' for the week to mark the occasion and the match more than lived up to expectations, despite the hot and humid conditions.
More to follow.
Line-ups
Gloucester-Hartpury: Sing; Rugman, Lund, Heard, Venner; George, Hunt (co-capt); Muir, Kelsey Jones, Delgado, Monaghan, Aldcroft (co-capt), Matthews, Lewis, Beckett.
Replacements: Powell, Buggy, Tuipulotu, Learned, Neve Jones, Blackburn, Bridger, Neumann.
Exeter: Doidge; Sinclair, Zackary, Cantorna, MacDonald; McGoverne, Robinson; Rogers, Tuttosi, Menin, Fryday, Leitch (capt), McMahon, Allen, Johnson.
Replacements: Moloney, Turani, Jacoby, Fleming, Jeffries, McDonald, Wilkins, Buchanan.