Wales head coach Wayne Pivac has brought in Hallam Amos, Owen Lane and Kieran Hardy as three changes to face Argentina on Saturday.
The backs trio replace Leigh Halfpenny, Tom Rogers and Tomos Williams from the side that defeated Canada 68-12.
Amos lines up at full-back in place of the injured Halfpenny, while Lane and Hardy replace Rogers and Williams, who drops to the replacements bench.
Pivac has named the same starting forward pack.
Halfpenny will require surgery after the full-back was ruled out of the summer series with a knee injury.
The 32-year-old suffered the injury within the first minute of his 100th Test appearance in the win against Canada.
Pivac explained why he was handing Cardiff Blues wing Lane his third cap at the expense of Rogers.
"His (Lane's) two Test matches were against Ireland in 2019 and then one at the World Cup, and on both occasions I thought he did really well," said Pivac.
"He hasn't had a consistent run, we left him out of the Canada game and just worked him hard on his fitness levels.
"He's worked hard over the last two-and-a-half weeks, so we're giving him an opportunity that he's going to relish.
"He's like a cat on a hot tin roof. With the injuries he's had, he's looking forward to this and hopefully he'll go well.
"Tom will be back in line for consideration next week. He enjoyed his first start and learned a lot.
"There were a couple of errors in his game he's been working on in training. I think a lot of the guys are finding that what they do in terms of preparation at club level, they need to be a little bit fitter at this level of the game.
"He's in that category with a lot of other young players."
There is one further change among the replacements, with Jarrod Evans recovering from the ankle injury that ruled him out from facing Canada and coming in for Ben Thomas.
Argentina warmed up for the first of two Tests against Wales with a narrow 24-17 victory over Romania in Bucharest.
"Argentina provide the perfect opponents for us this weekend and are the exact test we want for this group of players," added Pivac.
"We will be tested up front and the physicality will be at a level that some of these players have not experienced before, but that will answer a lot of questions for us as we build towards Rugby World Cup 2023.
"It's great to play in Cardiff once again and we want to play an exciting brand of rugby, which will have supporters up off their feet. It promises to be a great occasion."
Wales: Hallam Amos; Jonah Holmes, Willis Halaholo, Jonathan Davies (capt), Owen Lane; Callum Sheedy, Kieran Hardy; Nicky Smith, Elliot Dee, Dillon Lewis, Ben Carter, Will Rowlands, Ross Moriarty, James Botham, Aaron Wainwright.
Replacements: Ryan Elias, Gareth Thomas, Leon Brown, Josh Turnbull, Taine Basham, Tomos Williams, Jarrod Evans, Nick Tompkins.