Captain Owen Farrell has been released from isolation to rejoin the England squad after his Covid test was revealed to be a false positive.
However, the result was reviewed and, after subsequent tests proved negative, he is free to resume normal activities.
England Rugby say the process was "in line with advice given by UK Health Security Agency".
In Farrell's absence, George Furbank, more usually a full-back, started at 10 with Harlequins' Marcus Smith coming off the bench midway though the second half.
The possibility of head coach Eddie Jones naming Smith at 10 inside Farrell at inside centre had already been derailed by a minor leg injury that kept Smith out of training for much of the build-up.
That combination could now be likely for England's meeting with Australia next Saturday. Their autumn series concludes with a match against South Africa on 20 November.
It will be the first time the teams have met since South Africa's win in the 2019 Rugby World Cup final.